Negative emotions due to itch is a significant factor to diminish mental health in chronic itch patients. The amygdala was identified to be a key hub of network to regulate the negative emotions. However, the exact network remains unknown. This paper aimed to address this gap. We found that the amygdala network for processing itch involves the serotonergic system responsible for negative emotions and memory system to consolidate emotional experiences. We feel this finding is very important, because these systems may be­come therapeutic targets for preventing or reducing diminished mental health commonly seen in chronic itch patients.