Content » Vol 81, Issue 4

Investigative Report

Stereological Quantification of Lymphocytes in Skin Biopsies from Atopic Dermatitis Patients

Anne Ringer Ellingsen A1, Flemming Brandt Sørensen A2, Jytte Overgaard Larsen A3, Mette Søndergaard Deleuran A4, Kristian Thestrup-Pedersen A4
DOI: 10.1080/00015550152572886

Abstract

Atopic dermatitis (AD) is histologically characterized by lymphocytic infiltration of the skin and quantitative assessment is required. This study introduces stereological techniques to quantify the number of lymphocytes in skin biopsies. Fourmillimetre punch biopsies were taken from skin with active eczema in 8 adults with AD and from clinically normal skin from 4 of the patients. Five persons without allergy or skin disease served as controls. The mean number of lymphocytes in 4-mm skin biopsies was 469,000 and 124,000 in active eczema and in clinically normal skin, respectively. Compared with controls, the number of lymphocytes in biopsies increased by a factor of 6.8 in active eczema and a factor of 1.8 in clinically normal skin. If 20 % of skin is affected by eczema the total number of lymphocytes located in the affected skin can be estimated to 1.27 2 1010. A patient with clinically moderate AD has a consi

Significance

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