Content - Volume 69, Issue 2

Monoclonal antibodies were produced by immunizing mice with dispersed epidermal cells from a psoriatic plaque. We obtained 128 growing hybridoma clones. The immunohistochemical reactivity of the antibodies produced by these hybridomas was primarily screened on sections of skin biopsies from psoriatic plaques. Supernatants with positive staining were then tested on other dermatoses and human organs ...

We studied the cell infiltrates and antigenic characteristics of keratinocytes in biopsies from purpura pigmentosa chronica (PPC), with eleven monoclonal antibodies against several cell surface markers of effector and/or accessory cells of the immune system and compared the reactivity patterns with those in biopsies from uninvolved skin. Immunohistochemical staining revealed a predominance of acti ...

Precursor-product relationships among keratin polypeptides (cytokeratins) were studied in living and cornified layers of palmar-plantar epidermis. Serial 10 microns horizontal freeze-cut sections of punch biopsies were analysed by means of light microscopy and one-dimensional electrophoresis in a system that permitted identification of all major keratin polypeptides with apparent molecular weights ...

The lupus anticoagulant was found in six of 41 unselected patients with systemic lupus erythematosus (14%). Three of these six patients had episodes of thrombosis. Thrombosis occurred in only one patient in the remainder of the series without the lupus anticoagulant. The lupus anticoagulant should be considered as one of the criteria for the diagnosis of systemic lupus erythematosus, and it may be ...

It is generally accepted that cutaneous Lyme borreliosis comprises erythema chronicum migrans, lymphadenosis benigna cutis, and acrodermatitis chronica atrophicans. In recent years the tick-borne spirochete Borrelia burgdorferi has been associated with a number of other cutaneous disorders. We therefore investigated sera from 175 patients with localized scleroderma (morphea) (n = 64), systemic scl ...

A preparation procedure is presented for the determination of tyrosinase-catalysed stereo-specific dopa oxidase activity in serum. Purification is obtained by separation on a Phenyl-Sepharose hydrophobic interaction column, followed by Con-A-Sepharose chromatography. Five out of seven sera from patients with widespread melanoma metastases were found to contain detectable quantities of tyrosinase. ...

Tyrosinase was isolated from cultured melanoma cells using a procedure involving solubilization of the enzyme by means of Triton X-100, followed by different types of chromatography and tryptic digestion to make the enzyme soluble even in the absence of detergent. Starting with a membranous material containing 72 mg protein, 0.21 mg tyrosinase was obtained. The recovery of tyrosinase was 36% of th ...

The incidence of malignant melanoma of the skin has increased rapidly among white people during the last decade. Although the pathogenesis of malignant melanoma is not clear, epidemiologic data and experimental work indicates that ultraviolet (UV) radiation plays a critical role in tumorigenesis. Recent data implicate peroxidative reactions in UV-carcinogenesis and clearly demonstrate that seleniu ...

The effects of buthionine sulphoximine (BSO) treatment on cellular glutathione (GSH) content and on the cytotoxic action of menadione were investigated in cultured IGRI human melanoma cells. Addition of BSO (10(-8)-0.5 X 10(-3) M) to the cultures resulted in a dose- and time-dependent depletion of cellular GSH. BSO (10(-5) and 10(-6) M) did not influence cell multiplication up to 48 h, as determin ...

In order to evaluate the relative significance of previous grenz-ray treatment for human non melanoma skin carcinogenesis, the files were studied of all patients treated for non melanoma skin cancer of the scalp (n = 82, male/female ratio 1.1) at the Department of Dermatology, the Finsen Institute, from 1976 to 1985. Fourteen patients, with a male/female ratio of 3.7, were treated for squamous cel ...

In 10 in-patients with chronic plaque psoriasis, the antipsoriatic effect of MC903, a new synthetic analogue of vitamin D was evaluated. In each patient two symmetrical located psoriatic plaques were selected for the study. Topical treatment with MC903 cream (containing 1.2 mg MC903 per g cream) was compared with placebo cream in a double-blind, controlled, left-right, randomized way during 6 week ...

This is a study of the prevalences of chromate allergy and hand eczema among workers engaged in the manufacture of pre-fabricated concrete building components in Denmark in 1981 and again in 1987. In September 1981 the chromate content of cement manufactured and sold in Denmark was reduced to not more than 2 ppm (parts per million) of water-soluble chromate. This was accomplished by adding ferrous ...

Four members of a family, in which 8 suffered from diffuse palmoplantar keratoderma associated with an uncommon form of acrocyanosis, are reported. Acrocyanosis and palmoplantar keratoderma do not always occur together and, therefore, an autosomal dominant inheritance for this association is suggested.

Nine patients with atopic dermatitis (AD) were clinically evaluated before and after moving to new houses with improved air exchange, low relative humidity and optimal temperature control. During a 2-year period three clinical and subjective assessments were performed each month of disease activity, and compared with changes in suspended and respirable dust particles, room temperature, air exchang ...

There are several reports on the occurrence of immunoglobulin-producing B-cell lymphomas in S?©zary´s syndrome and mycosis fungoides, which may be the consequence of the helper activity of the neoplastic T-cells. Therefore we investigated skin biopsies of 50 patients with mycosis fungoides regarding the presence of plasma cells and their immunoglobulin profile. Nine of these patients had plasma ...

The clinical efficacy and transdermal absorption of permethrin, a new synthetic insecticide was investigated in ten scabies patients. All patients were successfully treated with one application of a cream, containing 5% permethrin. Apart from mild postscabies dermatitis no side-effects were observed. The mean weight of cream used per patient was 25 g (range 21-32; mean content of permethrin 1250 m ...

A peculiar form of late, superficial, serpiginous, non-ulcerative syphilides, leaving no visible scars, resembling clinically the ringworm of the glabrous skin, showing the granulomatous histopathological structure and relatively abundant T. pallidum, predominantly spread around the blood vessels is described.

Normal skin and psoriatic lesions from 35 patients were investigated immunohistochemically with regard to the CD1a+ cell population (Langerhans´ cells and indeterminate cells) in the epidermis as well as in the dermal infiltrate. In the normal-appearing skin, we found the regularly typical pattern of CD1a+ dendritic cells in suprabasal position, but in lesional skin of chronic psoriasis the CD1a+ ...

One hundred and five hirsute women were examined. Elevated levels of at least one androgen were found in 58 women, most often free testosterone, dehydroepiandrosterone sulfate and delta 4-androstenedione. No significant correlation was found between level of androgens and hirsutism score or frequency of menstrual disturbances other than amenorrhea. Fifty-three women were examined at least 6 months ...