Altered Monocytic Phenotypes are Associated with Uraemic Pruritus in Patients Receiving Haemodialysis
Mei-Ju Ko, Wan-Chuan Tsai, Yu-Sen Peng, Shih-Ping Hsu, Mei-Fen Pai, Ju-Yeh Yang, Hon-Yen Wu, Yen-Ling Chiu
Preview of fully accepted paper, still not published in any volume
Uraemic pruritus is one of the most bothersome symptoms in patients receiving haemodialysis. A total of 175 patients receiving maintenance haemodialysis, with 74 patients experiencing uraemic pruritus, were prospectively recruited to assess the influence of the phenotype of blood monocytes and various cytokines on uraemic pruritus. The phenotype of blood monocytes was determined by flow cytometry as classical (CD14++CD16−) monocytes, non-classical (CD14+CD16++) monocytes, and intermediate (CD14++CD16+) monocytes. Eight cytokines, including interleukin (IL)-2, interferon-γ, IL-12p70, IL-4, IL-5, IL-6, tumour necrosis factor-α, and IL-10, were simultaneously detected with a multiplex bead-based immunoassay. Multivariate linear regression analysis showed that a higher percentage of intermediate monocytes (effect estimate 0.08; 95% confidence interval 0.01–0.16) were independent predictors of a higher visual analogue scale score for pruritus intensity. No differences were noted for all 8 cytokines between patients with and without uraemic pruritus. The results of this study indicate that altered monocytic phenotypes could play a role in uraemic pruritus.
Uraemic pruritus is one of the most common and bothersome symptoms in patients receiving haemodialysis, but its pathophysiology remains obscure. Altered human monocyte phenotypes have been implicated in many chronic inflammatory diseases. This study found that a higher percentage of intermediate monocytes (CD14++CD16+) independently predicts a higher visual analogue scale score for pruritus intensity. Eight cytokines, including interleukin-2, interferon-γ, interleukin-12p70, -4, -5, -6, tumour necrosis factor-α, and interleukin-10, showed no differences between patients with and without uraemic pruritus. The results indicate that altered monocytic phenotypes could play a role in uraemic pruritus.