Content » Vol 62, Issue 5

Identification of mononuclear cells in cell suspension from cutaneous infiltrates freed by a suction blister method

Ahokas T, Niemi KM, Halme H.
DOI: 10.2340/0001555562377381

Abstract

Mononuclear cells were liberated from superficial skin inflammations by a suction blister method which yielded a sufficient number of cells for histochemical and immunocytochemical analysis. The cell population consisted mainly of T cells which were identified as sheep red blood cell rosettes. Very few B cells were found as rosettes with immunobeads. Macrophages and eosinophils were found in varying numbers and they were identified histochemically. The method is suitable for studying nontumorous skin infiltrates.

Significance

Supplementary content

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