Rapid assay of the anti-inflammatory activity of topical corticosteroids by inhibition of a UVA-induced neutrophil infiltration in hairless mouse skin. I. The assay and its sensitivity.
Woodbury RA, Kligman LH, Woodbury MJ, Kligman AM
A single large dose of UVA induced an intense infiltration of neutrophils into the lower dermis of hairless mouse skin, peaking at 24 h. The ability of 15 name brand topical corticosteroids to suppress this infiltrate was determined. The rank order of suppression correlated with the accepted clinical category of anti-inflammatory potency. This is a rapid screening procedure for assaying the anti-inflammatory activity of new steroids and for optimizing the vehicle.