Content - Volume 67, Issue 5

All articles

Substrate specific sulfatase activity from hair follicles in recessive X-linked ichthyosis
Dijkstra AC, Vermeesch-Markslag AM, Vromans EW, Happle R, van de Kerkhof PC, Zwanenburg B, Vos F, Vermorken AJ.
Recessive X-linked ichthyosis (RXLI) has its biochemical basis in a defect of the enzyme steroid sulfatase. Since several studies have reported a simultaneous deficiency of arylsulfatase C and steroid sulfatase it has been hypothesized that both enzymes are identical. In human hair follicles, however, hydrolytic activity for 4-methylumbelliferone sulfate, the substrate for arylsulfatase C, is foun ...
Pages: 369-376
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Ultrastructural aspects of skin after cryoultramicrotomy
Pautrat G, Bernerd F, Morel G, Ortonne JP, Hensby C.
Skin was studied by transmission electron microscopy after cryoultramicrotomy. Global and ultrastructural preservation of the tissue was obtained with satisfactory cohesion between dermis and epidermis. The main constitutive elements could be observed with good definition allowing easy recognition of all the organelles and therefore absolute identification of each cellular type encountered. The me ...
Pages: 377-384
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Membrane-coating granules in "dry" non-eczematous skin of patients with atopic dermatitis. A quantitative electron microscopic study
Werner Y, Lindberg M, Forslind B.
In recent years much interest has been focused on the functions of the membrane-coating granules (MCGs). These granules seem to play an essential role in the formation of the barrier of the stratum corneum by extruding their lipid-rich content into the extracellular space of the corneocytes. The dry non-eczematous skin in atopic dermatitis has been reported to have defective barrier function. In t ...
Pages: 385-390
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The detection of basal cell determinants in human basal cell carcinomas using two different monoclonal antibodies
Habets JM, Tank B, Vuzevski VD, van Reede EC, Stolz E, van Joost T.
This report deals with the reaction pattern(s) of two monoclonal antibodies (MoAbs) with normal skin and basal cell carcinomas (BCC). Using indirect immunoperoxidase (IIP) and indirect immunofluorescence (IIF) techniques, MoAb 12 G7 was observed to react with a determinant related to the cell membrane of the epidermal basal cells. In the IIP technique MoAb 12 G7 showed a positive reaction with 32 ...
Pages: 391-398
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Monoclonal antibody AHS-7 defines a specific basement membrane antigen localized to the hemidesmosome zone
Aiba S, Masuko T, Yagita H, Hashimoto Y, Tagami H.
MoAb AHS-7 labels the basement membrane of the skin. In this report, we have determined the precise localization and molecular nature of the antigen(s). After epidermal-dermal separation by suction blistering, the antigen(s) were detected on the epidermal side. Immunoelectron microscopy revealed the reaction product observed as a discontinuous line along the plasma membrane of the basal keratinocy ...
Pages: 399-405
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Keratinocyte T6 antigen expression after PUVA therapy
Dreno B, Milpied B, Stalder JF, Guillet G, Litoux P.
We report T6 antigen expression on keratinocytes in 11 cutaneous T lymphomas treated by PUVA therapy. This staining was absent before treatment. T6 reactivity was strictly limited to cell membrane. The nature of this expression is discussed, and it is suggested that it could be attributed to a passive diffusion from Langerhans´s cells.
Pages: 406-410
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Quantitative and morphologic changes of Langerhans´ cells after ultraviolet A irradiation of human epidermis
Liu HN, Muller SA, Schroeter AL.
Three different surface markers (OKT6, HLA-DR, and adenosinetriphosphatase) were compared to identify Langerhans´ cells, and the changes in number and morphology of these cells were studied at different intervals after irradiation of human skin by a 2.5-fold minimal erythema dose of ultraviolet A. Morphologic alteration and decreased surface-marker reactivity became evident on day 2 and were most ...
Pages: 411-416
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In vitro testing of contact sensitivity
Tjernlund U, Scheynius A, Strand A.
A new in vitro technique for the testing for contact sensitivity is described. The method is based upon the fact that in response to immunological stimuli, keratinocytes can start to synthesize and express class II histocompatibility antigens. Nickel sulphate (NiSO4) stimulated lymphocytes from nickel-sensitive persons, and the supernatant of these cells thus induced expression of HLA-DR on kerati ...
Pages: 417-421
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Production of interleukin-2 and interleukin-2 inhibitor in patients with palmoplantar pustulosis
Shiohara T, Kobayashi M, Ishii Y, Nagashima M.
We studied production of interleukin-2 (IL-2) and IL-2 inhibitor from peripheral blood of patients with palmoplantar pustulosis (PPP). Nineteen patients were divided into two groups: those with and those without arthro-osteitis. Although IL-2 production in both groups of patients was within normal limits, those with arthro-osteitis showed greater fluctuation in relation to the disease activity. Th ...
Pages: 422-426
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Leukocyte migration in vivo and in vitro in patients with psoriasis
Scheja A, Bruze M, Forsgren A.
Leukocyte migration in vivo was studied with a skin chamber technique in 21 patients with active psoriasis vulgaris and 18 with cleared psoriasis vulgaris. Measuring over 24 h, no difference was found between healthy volunteers and most patients with active psoriasis, although a subgroup of patients with long-lasting relapses showed subnormal migration values. In patients with cleared psoriasis on ...
Pages: 427-432
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Combination chemotherapy with bleomycin, cyclophosphamide, prednisone and etretinate (BCPE) in advanced mycosis fungoides: a six-year experience
Zachariae H, Thestrup-Pedersen K.
A six-year experience in 20 patients with advanced mycosis fungoides treated with combination chemotherapy with bleomycin, cyclophosphamide, prednisone and etretinate (BCPE) in advanced mycosis fungoides showed initial complete remissions in 16 patients (85%). The initial complete remissions lasted in average 8 months. A second complete remission was obtained in seven patients. The overall surviva ...
Pages: 433-437
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Delayed pressure urticaria syndrome: a clinical expression of interleukin 1
Shelley WB, Shelley ED.
Study of a patient with the bullous delayed pressure urticaria syndrome showed remarkable congruence of the extra-cutaneous findings and the known effects of interleukin 1: malaise, fever, myalgia, arthralgia, leukocytosis, increased sedimentation rate, and circulating acute phase reactants. As a result of this "clinical assay" for interleukin 1, we conclude that the delayed pressure urticaria syn ...
Pages: 438-441
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Analysis of increased urinary acid glycosaminoglycans in a patient with relapsing polychondritis
Tadaki T, Aiba S, Igarashi M, Tagami H.
We analysed the composition of glycosaminoglycans (GAGs) found in an increased amount in the urine from a patient with relapsing polychondritis (RP) by means of electrophoresis, since to our knowledge no analytical studies on the composition of GAGs in the urine from the patients with RP have been performed. Unexpectedly, the major GAGs detected in increased amount in the urine were found to be de ...
Pages: 441-445
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Experimental folliculitis with Pityrosporum orbiculare: the influence of host response
Goodfield MJ, Saihan EM, Crowley J.
The aetiology of the folliculitis associated with seborrhoeic eczema is unclear, though the yeast, Pityrosporum orbiculare has been implicated. P. orbiculare was applied under occlusion to normal forearm skin of patients with seborrhoeic eczema (SE), seborrhoeic eczema and folliculitis (SEF), and normal controls. There were significant differences in response to occlusion between the three groups. ...
Pages: 445-447
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Atopic dermatitis, house dust mite allergy and month of birth
Beck HI, Hagdrup HK.
An analysis of the month of birth in 210 patients with atopic dermatitis and a positive prick test towards the house dust mite (Dermatophagoides pteronyssinus) showed a significantly higher prevalence of birth in the interval May to November when compared with the expected distribution (p less than 0.01). A subdivision of the AD patients due to the concomitant presence of asthma showed no differen ...
Pages: 448-451
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Are acquired nevi oestrogen-dependent tumours?
Jemec GB, Bhogal BS, Wojnarowska F.
Twelve benign acquired nevi were studied for the possible specific binding of the ER D5 monoclonal antibody. The ER D5 monoclonal antibody identifies the p29 protein, which is found in the cytoplasm of oestrogen-sensitive cells and which is present in a higher concentration than nuclear oestrogen receptors. No staining was seen in nevoid cells, and the results are taken to support the hypothesis t ...
Pages: 451-453
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Common warts suggestive of allergy to metals: a case report
Kokelj F.
This report describes the case of a woman positive to nickel and cobalt patch tests and presenting common warts in the sites of contact with metals.
Pages: 453-455
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Pili torti and hirsuties: are twisted hairs a normal variant?
Barth JH, Dawber RP.
Twisted hairs as seen in pili torti have been observed on the abdomen and thighs of hirsute men and women. The occurrence of twisted hairs in persons without the syndrome of pili torti is discussed.
Pages: 455-457
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Tooth pits: an early sign of tuberous sclerosis
Requena Caballero L, Liron de Robles J, Requena Caballero C, Sánchez Yus E, Hernández Moro B.
The pit-shaped enamel defects seem to be pathognomonic of tuberous sclerosis and its detection may be an important help in the early diagnosis of this disease, especially in oligosymptomatic cases and when the dermatologic signs are still absent or only slightly evident.
Pages: 457-459
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