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Content - Volume 80, Issue 208

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Introduction
Hans Jörnvall, Rudolf Rigler
This article does not have an abstract.
Pages: 4-6
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Lipids and barrier function of the skin
Philip W. Wertz
The purpose of the present manuscript is to review the chemical and physical properties of epidermal lipids and to relate these properties to the formation and function of the permeability barrier of the skin. Lipids accumulate in small organelles known as lamellar granules as epidermal keratinocytes differentiate. This lipid is extruded into the intercellular spaces where it undergoes enzymatic p ...
Pages: 7-11
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Hypothesis: The epidermal permeability barrier is a porous medium
Neil Kitson, Jenifer L. Thewalt
The stratum corneum is a complex biological material characterized by very low permeability to water and most other molecules. This material may be thought of as a 'porous medium' composed of impermeable and permeable regions. Intercellular lipid membranes in the stratum corneum are postulated to exist in a mixture of two phases: solid (i.e. impermeable) and liquid crystalline (permeable). The co ...
Pages: 12-15
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Insights into the Molecular Organization of Lipids in the Skin Barrier from Infrared Spectroscopy Studies of Stratum Corneum Lipid Models
David J. Moore, Mark E. Rerek
In order to gain some insight into the molecular organization of lipids in the skin barrier we used Fourier transform infrared (FTIR) spectroscopy to investigate models of the stratum corneum (SC) containing deuterated hexadecanoic acid, cholesterol, and ceramide 2 or ceramide 5. In both models there is clear evidence of separate conformationally ordered domains of ceramide and fatty acids. In add ...
Pages: 16-22
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The Lipid Organisation in the Skin Barrier
J. A. Bouwstra, F. E. R. Dubbelaar, G. S. Gooris, M. Ponec
The main function of the skin is to protect the body against exogenous substances. The skin barrier is located in the outermost layer of the skin, the stratum corneum. This layer consists of keratin enriched cells embedded in lipid lamellae. These lamellae form the main barrier for diffusion of substances through the skin. In diseased skin the barrier function is often impaired. For a full underst ...
Pages: 23-30
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The skin barrier from a lipid perspective
Sven Engström, Katarina Ekelund, Johan Engblom, Liselotte Eriksson, Emma Sparr, Håkan Wennerström
This contribution summarises the results from a number of investigations undertaken in the spirit of the Domain Mosaic Model proposed by Forslind in 1994. Atomic Force Microscopy (AFM) studies on the two-dimensional phase behaviour of some stratum corneum lipids revealed phase separation of the lipids in the typical case and the ability of cholesterol to reduce the line tension between phases. A ...
Pages: 31-35
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Visualization of percutaneous 3H-estradiol and 3H-norethindrone acetate transport across human epidermis as a function of time
Jan A. M. Neelissen, Christoph Arth, Michael Wolff, Ad H. G. J. Schrijvers, Hans E. Junginger, Harry E. Boddé
Developing transdermal therapeutic systems for estradiol and norethindrone acetate raised questions about the steroids penetration pathway across and retention in the skin. This paper describes the distribution of 3H-estradiol and 3H-norethindrone acetate in human stratum corneum after topical application to dermatomed skin in vitro. The study involved (a) permeation e ...
Pages: 36-43
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Desquamation in the Stratum Corneum
Torbjörn Egelrud
To maintain a constant thickness of the stratum corneum the desquamation rate and the de novo production of corneocytes is delicately balanced. Using a plantar stratum corneum model we have obtained evidence that proteolysis is a central event in the desquamation process. A number of regulatory mechanisms for desquamation have been postulated based on our findings.
Pages: 44-45
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The skin barrier: analysis of physiologically important elements and trace elements
Bo Forslind
Changes in the properties of the skin barrier should have correlates in the physiological status of the differentiating epidermal cells. However, the quantitative distributions of physiologically important elements and trace elements of the skin has been a neglected area of research for lack of tools to investigate this highly differentiated tissue. With the event of the particle probes, the elect ...
Pages: 46-52
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