Content » Vol 93, Issue 3

Letter to the Editor

Allogeneic Haematopoietic Stem Cell Transplantation in a Patient with Cutaneous γ/δ-T-cell Lymphoma

Sarah Terras1#, Rose K. C. Moritz1#, Markus Ditschkowski2, Dietrich W. Beelen2, Peter Altmeyer1, Markus Stücker1 and Alexander Kreuter1*

1Department of Dermatology, Venereology and Allergology, St Josef Hospital Bochum, Ruhr University Bochum, Gudrunstr. 56, DE-44791 Bochum, and 2Department of Bone Marrow Transplantation, West German Cancer Center, University Hospital Essen, Essen, Germany. *E-mail:

Accepted June 26, 2012.

#These authors contributed equally to this paper and should be considered as first authors.

Reported cases of subcutaneous panniculitis-like T-cell lymphoma (SPTL) show different clinical courses depending on the immunophenotype of the tumour cells. They can either express α and β or γ and δ polypeptide chains in the T-cell receptor (TCR). These differences caused the World Health Organization’s European Organization for Research and Treatment of Cancer (WHO-EORTC) to classify this type of lymphoma into an α/β-TCR subtype (SPTL-AB) solely constituting the group of panniculitis-like T-cell lymphoma, and a γ/δ-TCR subtype (CGD-TCL), which was regrouped into the broad category of peripheral T-cell lymphoma, not otherwise specified (1).

Clinically, CGD-TCL appears as indurated red plaques and nodes with predominance to the trunk and the extremities often leading to diagnostic confusion with differential diagnosis such as lupus panniculitis, cellulitis or other erythematous skin diseases. Ulcerations have rarely been reported.


A 51-year-old woman presented with a 10-month history of partly ulcerating painful subcutaneous nodules and plaques with predominance of the trunk and the extremities (Fig. 1 a, b). She did not report any B-symptoms. The patient’s medical history revealed chronic seronegative rheumatoid polyarthritis, myocardial infarction at the age of 45 years, arterial hypertension, lung emphysema, allergic rhinoconjunctivitis and substituted hypothyroidism. Blood count on first presentation showed moderate leucopaenia and neutropaenia (leukocytes: 3,360/µl (normal 4,600–9,500), neutrophils: 1,610/µl, (1,800–7,200)), without elevated lymphocyte count (1,470 (1,000–4,050)).

A representative histological specimen showed a superficial and deep angiocentric infiltrate of medium- to large-sized pleomorphic lymphocytes with focal “fat-cell-rimming” as well as necrosis of the dermis and subcutis (Fig. 1c). Immunohistochemical staining revealed a CD4-, CD8- and CD56-negative infiltrate, whereas granzyme B, and especially Ki67 showed high positivity (Fig. 1d). Beta-F1 staining was negative in 80% of the tumour cells. Furthermore, we found a positive TCR-γ-rearrangement in the monoclonal lymphocytes, while TCR-δ-rearrangement was lacking. In situ hybridization for Epstein Barr virus (EBER) was negative in the tumour material. A γ/δ-T-cell lymphoma was diagnosed with regard to the histological appearance and the immune profile (Table SI; available from according to the EORTC classification of T-cell lymphomas (2).


Fig. 1. (a) Deep lymphocytic infiltrate and (b) highly positive Ki67-staining. (c, d) Red patches, plaques and partly ulcerating nodules on initial presentation, and (e, f) completely regressive skin lesions with remaining ulcerations on the right ankle healing well 18 months after allogeneic haematopoietic stem cell transplantation.

Staging included computed tomography scan of the chest and abdomen, sonography of the lymph nodes, bone marrow smear, bone marrow biopsy, and peripheral blood smear, all showing no signs of lymph node, organ or bone marrow involvement. The resulting tumour-node-metastasis (TNM)-classification before treatment according to the EORTC was T3N0M0 at first presentation (1).

Our patient underwent combined topical psoralen and ultraviolet A (PUVA)-therapy, as well as treatment with metho­trexate and systemic corticosteroids, all with no response. After establishment of the diagnosis of CGD-TCL, monotherapy was started with 800 mg/m² body surface gemcitabine over 2 cycles, followed by a monochemotherapy with 100 mg/m² body surface bendamustine over 3 cycles. Both led to no clinical improvement.

With regard to the limited prognosis of the CGD-TCL, the relatively young age, with regard to the type of her disease, and the patient’s otherwise reasonable medical status (Eastern Cooperative Oncology Group (ECOG) performance status: 1) we took allogeneic stem cell transplantation (SCT) into consideration. The tumour stage before transplantation was T3N0M0 according to the EORTC. A non-related HLA-identical donor could be identified. Pre-transplant myeloablative conditioning included 175 mg fludarabine, 200 mg busulfan and 5,800 mg cyclophosphamide. For immunoprophylaxis, cyclosporine (according to serum level, starting with 2 mg/kg body weight intravenously) and a short course of methotrexate, 15 mg/m2 body surface, were used. We avoided performing in vivo T-cell depletion or administering a T-cell depleted graft in order to permit a possible T-cell mediated graft-vs.-lymphoma reaction. The patient received peripherally collected hematopoietic progenitor cells at a concentration of 9.6 × 106/kg body weight CD34-positive cells.

Complete remission of symptoms of disease, with regression of plaques and nodes as well as the continuous healing of ulcerations, was noted after allogeneic SCT (Fig. 1 e, f). The patient developed acute and subsequently secondary chronic cutaneous graft vs. host disease (GvHD) that could be controlled by systemic immunosuppressive treatment with cyclosporine 35 mg daily and prednisone 10 mg daily. Other complications comprised viral infections. BK-virus-(human polyomavirus 1)-induced cystitis and an ocular herpes simplex infection were observed during the early post-transplant course and were treated successfully by virostatic therapy. After a follow-up of 21 months the patient is in complete remission with no signs of disease recurrence. She has severe chronic GvHD with skin and lung involvement, which is currently under good control with moderate inflammatory activity. Scoring of chronic GvHD was performed by National Institute of Health (NIH) criteria (3). Pulmonary GvHD was ascertained 8 months after SCT by pulmonary function test and radiology. Severe impairment of forced expiratory volume (FEV1) to 15% of normal values was found, as defined by bronchiolitis obliterans (BO). By the use of anti-obstructive treatment and increased immunosuppression the course of BO remained stable. The patient was last seen in January 2012.


CGD-TCL is associated with an often fatal prognosis (5-year overall survival: 11%). In our case, no B-symptoms (fever, weight loss and night sweats) or organ involvement were present. Nevertheless, cutaneous involvement was progressing steadily, with poorly healing ulcerating nodes, and was refractory to any treatment.

To date, there is no established therapy for CGD-TCL and treatment guidelines are mostly based on anecdotal reports (4). Most treatments consisted of multi-agent doxorubicin-based chemotherapies (mostly CHOP) as first- or second-line treatment, as well as prednisone and autologous or allogeneic SCT in case of progressive disease. Narrow-band ultraviolet radiation (NB-UVB) and low-dose methotrexate have been shown to be a useful treatment in the patch/plaque lesions of primary CGD-TCL, especially in elderly people (5). Bexarotene has been used alone, in combination with, or as maintenance therapy after, CHOP/CHOP-like regimen in the treatment of SPTL-AB and CGD-TCL (6). Hathaway et al. (7) reported a case of a patient with CGD-TCL with partial remission under denileukin diftitox and complete response after its combination with bexarotene.

Willemze et al. (2) reported one case of CGD-TCL with complete remission after allogeneic SCT. In a meta-analysis Go & Wester (8) report that after treatment with high-dose chemotherapy and stem cell transplantation for refractory or recurrent disease, a complete remission was achieved in 92% of all cases, with a median response duration of 14 months. A distinction between SPTL-AB and CGD-TCL was lacking in this meta-analysis, which was published prior to the new EORTC classification. Yuan et al. (9) reported one case with relapse of disease in a patient with CGD-TCL after allogeneic SCT and complete remission after withdrawal of immunosuppressive treatment (cyclosporine). This underlines the possibility of a graft-vs.-T-cell lymphoma effect, which has been described for non-T-cell lymphomas and leukaemia (2, 10). Table SII (available from summarizes the previously published cases.

Selecting the best timing for allogeneic haemato­poietic stem cell transplantation in patients with T-cell lymphomas is often difficult. Radical therapies are rarely accepted by both patient and practitioner as long as the symptoms of disease do not appear life-threatening. Yet a good physical condition is essential for a favourable post-transplant outcome. Early recognition of possible candidates for allogeneic SCT and individualized, toxicity-reduced conditioning regimens, combined with modern concepts of immunoprophylaxis, could contribute to minimize transplant-related mortality.

The authors declare no conflicts of interest.


1. Kim YH, Willemze R, Pimpinelli N, Whittaker S, Olsen EA, Ranki A, et al. TNM classification system for primary cutaneous lymphomas other than mycosis fungoides and Sezary syndrome. Blood 2007; 110: 479–484.

2. Willemze R, Jansen PM, Cerroni L, Berti E, Santucci M, Assaf C, et al. Subcutaneous panniculitis-like T-cell lymphoma: definition, classification, and prognostic factors: an EORTC Cutaneous Lymphoma Group Study of 83 cases. Blood 2008; 111: 838–845.

3. Lee S, Cook EF, Soiffer R, Antin JH. Development and validation of a scale to measure symptoms of chronic graft-versus-host disease. Biol Blood Marrow Transplant 2002; 8: 444–452.

4. Guitart J. Subcutaneous lymphoma and related conditions. Dermatol Ther 2010; 23: 350–355.

5. Fujii M, Uehara J, Honma M, Ito Y, Takahashi H, Ishida-Yamamoto A, et al. Primary cutaneous gammadelta-T-cell lymphoma treated with low-dose methotrexate and narrowband ultraviolet B irradiation: report of a case with testicular involvement. J Dermatol 2011; 38: 368–372.

6. Mehta N, Wayne AS, Kim YH, Hale GA, Alvarado CS, Myskowski P, et al. Bexarotene is active against subcutaneous panniculitis-like T-cell lymphoma in adult and pediatric populations. Clin Lymphoma Myeloma Leuk 2010; 12: 20–25.

7. Hathaway T, Subtil A, Kuo P, Foss F. Efficacy of denileukin diftitox in subcutaneous panniculitis-like T-cell lymphoma. Clin Lymphoma Myeloma 2007; 7: 541–545.

8. Go RS, Wester SM. Immunophenotypic and molecular features, clinical outcomes, treatments, and prognostic factors associated with subcutaneous panniculitis-like T-cell lymphoma: a systematic analysis of 156 patients reported in the literature. Cancer 2004; 101: 1404–1413.

9. Yuan L, Sun L, Bo J, Zhou Y, Li HH, Yu L, et al. Durable remission in a patient with refractory subcutaneous panniculitis-like T-cell lymphoma relapse after allogeneic hematopoietic stem cell transplantation through withdrawal of cyclosporine. Ann Transplant 2011; 16: 135–138.

10. Weiden PL, Flournoy N, Thomas ED, Prentice R, Fefer A, Buckner CD, et al. Antileukemic effect of graft-versus-host disease in human recipients of allogeneic-marrow grafts. N Engl J Med 1979; 300: 1068–1073.

11. Magro CM, Crowson AN, Kovatich AJ, Burns F. Lupus profundus, indeterminate lymphocytic lobular panniculitis and subcutaneous T-cell lymphoma: a spectrum of subcuticular T-cell lymphoid dyscrasia. J Cutan Pathol 2001; 28: 235–247.

12. Przybylski GK, Wu H, Macon WR, Finan J, Leonard DG, Felgar RE et al. Hepatosplenic and subcutaneous panniculitis-like gamma/delta T cell lymphomas are derived from different Vdelta subsets of gamma/delta T lymphocytes. J Mol Diagn 2000; 2: 11–19.

Supplementary content
Table SI
Table SII